Metachromatic Granules Stain
Principle:
The size of Corynebacterium diphtheriae is very variable, essentially rod-shaped but often showing irregular expansion at one end. Cells frequently lie in small clusters at acute angles to each other in V, L or Y shapes. Gram positive but readily decolorized, non-motile, non-capsulate, non-sporing.
Strains are distributed as beans and stained unevenly. With Metachromatic Granules Stain, deeply stained granules known as metachromatic granules, are found on one side or both sides of diphtheriae.
Specifications:
|
Contents |
2vials×20ml/Kit |
Components |
|
Solution A |
1vial×20ml |
Toluidine Blue |
|
Solution B |
1vial×20ml |
Potassium iodide |
Methods:
1. Fix smears on flames, add solution A to stain for 3~5 minutes and rinse with water.
2. Add solution B to stain for 1 minute and rinse with water.
3. Dry and observe microscopically.