Naphthol AS-D Chloroacetate Esterase (AS–DCE) Stain
Intended Use:
This kit is for staining bone marrow cell and bloodcell smear.
Principle:
Naphthol AS-D chloroacetate can be hydrolyzed into Naphthol AS-D by Esterase to mix with diazonium salt and form insoluble red-brown precipitate in cytoplasm. Positive reaction is only found in granulocytes; hence, it is also called "Specific Esterase Stain".
Specifications:
| Contents | 5Tests/Kit | 20Tests/Kit | 100Tests/Kit | Components |
| Fixative (solution A) | 1vial*2.5m | 1vial*10ml | 1vial*50ml | Sodium Citrate |
| Pararosaniline (solution B) | 1vial*0.1ml | 1vial*0.3ml | 1vial*1.1ml | Parafuchsin |
| Sodium Nitrite (solution C) | 1vial*0.1ml | 1vial*0.3ml | 1vial*1.1ml | Sodium Nitrite |
| Phosphate Buffer (solution D) | 1vial*5ml | 1vial*20ml | 1vial*95ml | Phosphate |
| Naphthol As-D Chloroacetate (solution E) | 1vial*0.3ml | 1vial*1.2ml | 1vial*5.5ml | Naphthol AS-D hloroacetate |
| Methyl Green (solution F) | 1vial*5ml | 1vial*20ml | 1vial*100ml | Methyl Green |
Methods:
Working solution Preparation(for 1 test only);
Apparatus required: disposable tube, micropipette, disposable tips, dropper;
Instruction: Mix 10µl solution B and10µl solution C thoroughly. Wait for 1 Minute;
Add 0.9ml solution D and 50µl solution E. Mix well;
Expected Results:
Red granules found in cytoplasm means positive.