Neutrophilic- Leukocyte Alkaline Phosphatase (NAP) Stain
Intended Use:
This kit is for staining bone marrow cell and bloodcell smear.
Principle:
Neutrophilic-Leukocyte Alkaline Phosphatase stain is based on diazotization-coupling principle. At pH9.2~9.8, Alkaline Phosphatase hydrolyzes Naphthol AS-BI Phosphoric Acid into α-naphthol. The stable diazonium salt and naphthol shall form insoluble colored precipitate in cytoplasm.
Specifications:
| Contents | 5Tests/Kit | 20Tests/Kit | 100Tests/Kit | Components |
| Fixative (solution A) | 1vial*5ml | 1vial*20ml | 1vial*100ml | Sodium Citrate |
| FBB -Alkaline (solution B) |
1vial*0.3ml | 1vial*1.1ml | 1vial*5.1ml | Fast Blue BB Base |
| Sodium Nitrite (solution C) |
1vial*0.3ml | 1vial*1.1ml | 1vial*5.1ml | Sodium Nitrite |
| Naphthol AS-Bl Alkaline (solution D) |
1vial*0.3ml | 1vial*1.1ml | 1vial*5.1ml | Naphthol AS-Bl |
| Neutral Red (solution E) |
1vial*5ml | 1vial*20ml | 1vial*100ml | Neutral Red |
Methods:
Working solution Preparation (for 1 test only)
Apparatus required:disposable tube, micropipette, disposable tips, dropper;
Instruction:Mix 25µl solution B with 25µl C; wait for 2 minutes. Add distilled water 2ml into 25µl solution D. Mix thoroughly.
Expected Results:
NΑP positive granules are blue.